Replacing Helper Lipids With Cationic Lipids Enhances mRNA Lipid Nanoparticles Stability in Solution.

ABSTRACT: Messenger RNA lipid nanoparticles (mRNA-LNPs) have achieved remarkable success in clinical vaccination efforts to curb the COVID-19 pandemic, and have attracted tremendous interest from both industry and academia to broaden their biomedical applications. However, their storage and transportation rely heavily on the cold chain to prevent rapid degradation in non-frozen solutions, posing significant challenges for logistics and accessibility. Therefore, enhancing the in-solution stability of mRNA LNPs is crucial. Herein, the role of helper phospholipids is investigated in storage stability of mRNA LNPs. By ratiometrically replacing the helper phospholipids (DSPC) with other alternative lipids (DOPS, DOTAP, and DOPE) in commercially available mRNA LNP formulation, enhanced in-solution stabili

SUBMITTER: Chen R 

PROVIDER: S-EPMC12806310 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature