An Efficient CO₂-Upcycling Platform Based on Engineered Halomonas TD with Enhanced Acetate-Utilizing Capacity via Adaptive Laboratory Evolution.

ABSTRACT: Biohybrid conversion of carbon dioxide (CO₂) into value-added bioproducts via engineered microbes using CO₂-derived electrolytes (CDE) addresses global CO₂ emissions, but most recombinants have poor saline CDE tolerance and low carbon conversion rate (CCR). Herein, Halomonas TD (salt-resistant) was adaptively evolved into TD80, which efficiently uses acetate; its aceE gene mutation (encoding pyruvate dehydrogenase) drives acetate utilization. Subsequently, different biosynthesis pathways in TD80 enabled high yields of poly-3-hydroxybutyrate (PHB), poly-3-hydroxybutyrate-co-4-hydroxybutyrate (P34HB), 3-hydroxybutyrate (3HB), violacein, ectoine, 1,3-diaminopropane (1,3-DAP) and superoxide dismutase (SOD), respectively. Moreover, 26.0 g L^-1 ectoine

SUBMITTER: Wang C 

PROVIDER: S-EPMC12806439 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature