High performance charge detection mass spectrometry without ultra-high vacuum.

ABSTRACT: Charge detection mass spectrometry (CDMS) measurements of individual ions using either Orbitrap or electrostatic ion trap-based instruments have heretofore been performed under ultra-high vacuum conditions (10^-9 Torr or lower). The rationale for this expensive and often cumbersome requirement is that these measurements need to be performed in an environment where collisions with background gas do not adversely affect the measurements. Here, the use of an electrostatic trap that accepts a broad range of ion energies and a dynamic ion signal analysis method enables accurate CDMS mass measurements at pressures as high as 1 × 10^-6 Torr, multiple orders of magnitude higher than previously demonstrated. Consistent, accurate masses were obtained for pentameric antibody compl

SUBMITTER: Hanozin E 

PROVIDER: S-EPMC12807536 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature