RMPore: a comprehensive database of single-molecule RNA modifications detected by Nanopore direct RNA sequencing.

ABSTRACT: Transcriptome profiling of RNA modifications is essential for uncovering and characterizing novel post-transcriptional regulatory mechanisms. However, most of the known RNA modifications remain poorly explored due to technological limitations. Nanopore direct RNA sequencing (DRS) provides an advantageous solution for transcriptome-wide RNA modification profiling, enabling simultaneous identification of any modification type in native RNA, with full-length coverage and single-molecule resolution. We developed RMPore (https://rmpore.renlab.cn/), a comprehensive database of single-molecule RNA modifications detected from 958 DRS samples across 34 species. We constructed a practical analytical pipeline integrating 20 detection tools and categorized all detected sites into three confidence leve

SUBMITTER: Lin Z 

PROVIDER: S-EPMC12807678 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature