ConnectomeDB2025: a rigorously curated, multi-species resource of experimentally supported ligand-receptor interactions.

ABSTRACT: Inferring cell-cell communication networks is now a cornerstone of single-cell RNA-seq and spatial transcriptomics data analysis, relying critically on reference catalogues of experimentally supported ligand-receptor interactions. Here, we present the updated, rigorously curated connectomeDB, an open-access database of peptide-based ligand-receptor pairs comprising 3579 vertebrate interactions supported by primary experimental evidence from 2803 research articles. By critically reviewing all putative ligand-receptor pairs from connectomeDB2020, CellChatDB v2, CellPhoneDB v5, CellTalkDB, ICELLNET v2, and LIANA+, we first removed over 2900 misclassified or unsupported interactions lacking primary-literature evidence. We then expanded the resulting verified dataset through AI-assisted literat

SUBMITTER: Liu P 

PROVIDER: S-EPMC12807709 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature