PhaSepDB 3.0: a comprehensive knowledgebase of phase separation-related proteins from AI-assisted curation.

ABSTRACT: Phase separation (PS) is a fundamental principle driving the formation of membraneless organelles (MLOs), which are critical for various cellular functions and pathological conditions. We present PhaSepDB 3.0 (https://db.phasep.pro/), a significantly updated knowledgebase of proteins related to PS. To address the challenges of curating a vast body of literature, we have implemented a novel human-AI collaborative workflow that integrates a large language model (LLM)-based agentic system with expert verification, enabling a major expansion and enrichment of the database. PhaSepDB 3.0 now contains 3,484 expert-curated entries for 1849 PS-related proteins, more than doubling the content of the previous version. The annotation framework has been restructured to capture deeper insights, includin

SUBMITTER: You K 

PROVIDER: S-EPMC12807760 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature