HPV-driven transcriptome and splicing rewiring under SRPK1 inhibition in cervical cancer.

ABSTRACT:

Background

Serine/arginine protein kinase 1 phosphorylates serine-arginine-rich (SR) proteins to regulate splice-site selection during alternative splicing. While its role in general RNA regulation is established, its contribution to the HPV-dependent transcriptome and splicing stratification in cervical cancer remains unclear. Therefore, we sought to determine how SRPK1 inhibition differentially remodels gene expression and alternative splicing in HPV⁺ versus HPV^- cervical cancer cells.

Methods

HPV16⁺ SiHa and HPV^- C33A cervical cancer cells were treated with the SRPK1 inhibitor, SPHINX31. RNA profiling was performed, and differentially expressed genes were defined as |log₂FC| ≥ 1.5. AS events were classified by SUPPA a

SUBMITTER: Basera A 

PROVIDER: S-EPMC12807931 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature