Single-cell RNA sequencing unraveled immune-related expression heterogeneity and lymphoid cell development dysregulation in childhood asthma.

ABSTRACT:

Backgrounds

Asthma is a chronic inflammatory disease affecting airways, usually starting in childhood. Its cause remains unclear.

Objective

We aim to elucidate the role of immune dysregulation in the pathogenesis of pediatric asthma.

Methods

In this study, we used single-cell RNA sequencing to analyze peripheral blood mononuclear cells (PBMCs) from three pediatric asthma patients and four age-matched healthy controls to investigate the cellular etiology of childhood asthma.

Results

The overall expression patterns of PBMCs from the three asthma patients indicate that both innate and adaptive immunity are imbalanced and abnormally activated in childhood asthma. Analysis of hematopoietic stem and progenitor cells (HSPCs) expression profiles further reveals that HS

SUBMITTER: Zhu D 

PROVIDER: S-EPMC12807962 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature