Integrative proteomics and metabolomics analysis of the mechanism of pancreatic β-cell dysfunction in aged mice.

ABSTRACT:

Objectives

The prevalence of type 2 diabetes mellitus has increased worldwide and is higher among older individuals. Exploring the mechanisms underlying pancreatic β-cell dysfunction may help elucidate the pathogenesis of age-related diabetes.

Methods

Islet function-related parameters were measured in four young and four aged mice. Endogenous proteins and metabolites in the pancreas were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics and metabolomics, and integrated data analysis was performed.

Results

Compared with young mice, aged mice presented higher fasting blood glucose levels and insulin resistance index (according to the homeostatic model assessment for insulin resistance, HOMA-IR), whereas that from the homeostasis m

SUBMITTER: Pan F 

PROVIDER: S-EPMC12807971 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature