Sex-specific effects of peptidyl arginine deiminase 4 deficiency in the cafeteria diet-induced obesity-associated metabolic complications.

ABSTRACT:

Background

Obesity is a global health challenge linked to chronic non-communicable diseases. Low-grade inflammation and altered immune responses, including neutrophil extracellular trap (NET) formation, contribute to metabolic complications. Peptidyl arginine deiminase 4 (PAD4) is critical for NET formation, and its inhibition shows therapeutic potential. However, the sex-specific effects of PAD4 deficiency in diet-induced obesity remain unexplored.

Methods

This study investigated the impact of PAD4 deficiency on obesity-related metabolic pathologies in male and female C57BL/6 wild-type (WT) and Pad4^(-/-) mice (n=5-6/group) fed a 22-week obesogenic cafeteria (CAF) diet. We hypothesized that PAD4 deficiency would ameliorate obesity-related metabolic and behavioral

SUBMITTER: Fejes A 

PROVIDER: S-EPMC12807990 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature