Integrating flow cytometric profile and gene mutation analysis of intact lymph nodes to refine lymphoma immunophenotype.

ABSTRACT:

Objective

To evaluate the diagnostic use of integrating multi-parameter flow cytometry (FCM), histopathology, and gene mutation analysis for lymphoma classification using intact lymph node (LN) samples.

Methods

Intact LN samples from 109 patients with lymphadenopathy were retrospectively analyzed by pathology, multi-color FCM, and next-generation sequencing (NGS) targeting 62 lymphoma-related genes.

Results

FCM immunophenotyping showed high concordance with pathology (56/56 lymphoma cases detected by FCM were pathologically confirmed). PD-1 on T cells was significantly elevated in B-cell lymphoma (BCL), especially diffuse large BCL (DLBCL) (P<0.05). All BCLs exhibited monotypic intracellular cKappa or cLambda expression. Significant differences in cell size (FS

SUBMITTER: Peng QY 

PROVIDER: S-EPMC12808046 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature