Single-molecule microtubule dynamics measurements reveal an intermediate state and clarify the role of nucleotide.

ABSTRACT: Microtubules are polymers of αβ-tubulin heterodimers that undergo GTPase dependent polymerization dynamics to organize the intracellular space and mediate faithful chromosome segregation. Microtubule dynamics derive from biochemical properties of individual tubulin subunits and how they interact with the polymer end, a complex environment where individual tubulins can have different numbers of neighbor contacts. A fundamental understanding of microtubule dynamics has been difficult to establish because of challenges measuring the strength, nucleotide-dependence, and proportion of different tubulin binding sites on the microtubule end. Using an improved single-molecule assay to measure tubulin:microtubule interactions, we discover that tubulin can isomerize to bind more tightly to the micro

SUBMITTER: Mondal S 

PROVIDER: S-EPMC12808227 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature