Comparison of classic Sanger and next generation sequencing mitotypes of second world war victims from Konfin I mass grave.

ABSTRACT: Rapid technological advancements have significantly enhanced DNA analysis. A key innovation is Next-Generation Sequencing (NGS), also known as Massively Parallel Sequencing (MPS), which followed classic Sanger (CS) sequencing. Compared to CS, NGS offers higher sensitivity, resolution, and throughput, making it particularly valuable for mitochondrial DNA (mtDNA) analysis. The high copy number, matrilineal inheritance, and non-recombining nature of mtDNA, especially its hypervariable regions (HV), make it highly relevant in forensic investigations. NGS has introduced streamlined protocols and improved low-level heteroplasmy detection in mtDNA sequencing. However, with any new technology, its informativeness and authenticity must be evaluated against traditional methods. This study compared m

SUBMITTER: Obal M 

PROVIDER: S-EPMC12808251 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature