Identification of key genes related to metabolic cell death in hepatic ischemia-reperfusion injury from transcriptome data and mechanism research using single-cell data.

ABSTRACT:

Background

Ferroptosis and cuproptosis are closely associated with hepatic ischemia-reperfusion injury (HIRI). However, the significance of metabolic cell death-related genes (MRGs) in HIRI still awaits exploration. This study examined the molecular mechanisms through which key genes contribute to metabolic cell death in HIRI.

Methods

GSE12720, GSE14951, and GSE171539 datasets and 478 MRGs were included. First, candidate genes were screened through differential expression analysis combined with MRGs. Then, key genes were identified by using machine learning algorithms combined with expression verification. Subsequently, the analyses included constructing and evaluating nomograms, conducting functional enrichment studies, characterizing immune infiltration, building regulator

SUBMITTER: Yu H 

PROVIDER: S-EPMC12808398 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature