Busulfan inhibits <i>Pseudomonas aeruginosa</i> growth and reduces biofilm biomass and pyocyanin production.

ABSTRACT:

Background

Pseudomonas aeruginosa remains a serious threat in clinical settings, especially among patients who are immunocompromised, receiving chemotherapy, or in intensive care units. With the rise of antibiotic resistance, drug repurposing offers a promising alternative strategy. Busulfan, an anticancer alkylating agent that induces DNA cross-linking and cytotoxic effects in cancer cells, may exert similar effects on microorganisms, as reported for other alkylating agents.

Methods

This study evaluated the antibacterial potential of busulfan against P. aeruginosa. Initially, the antimicrobial activity of busulfan was assessed using the microdilution method, followed by investigations of key virulence factors of the PAO1 strain after treatment.

Results

SUBMITTER: Bahkali NA 

PROVIDER: S-EPMC12808452 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature