Molecular crosstalk between AHR, CYP1B1, and ITGAM in diabetic nephropathy: Integrated insights from bioinformatics and experimental validation.

ABSTRACT: Chronic inflammation and a progressive loss of kidney function are hallmarks of diabetic nephropathy (DN). Th22 cells are mainly regulated by the transcription factor AHR, which is essential for their differentiation and inflammatory responses. This study aims to investigate the regulatory role of Th22 cells in the progression of DN. The GEO database was used to retrieve the GSE142025 (DN) and GSE135390 (Th22) datasets, which provided transcriptomic profiles for gene expression analysis. WGCNA was employed to identify gene co-expression modules strongly correlated with DN progression. Gene expression levels were validated by real-time PCR in 90 PBMC samples (30 per group: T2DM, DN, and healthy controls). Gene-drug interactions were subsequently predicted using the DGIdb database. In DN, 40

SUBMITTER: Hosseini SA 

PROVIDER: S-EPMC12808505 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature