A CRISPR-assisted passive microfluidic chip for rapid, visual detection of multiple respiratory viruses.

ABSTRACT: In recent years, viral co-infections, particularly with respiratory viruses, have resulted in more complex symptoms, a greater disease burden, and increased challenges in clinical decision-making. These complexities underscore the urgent need for improved diagnostic tools in the managing acute respiratory infections. To address the limitations of conventional qPCR and current POCT methodologies, we developed a passively driven microfluidic chip capable of rapidly screening multiple respiratory viruses. This platform is particularly suited for the point-of-care diagnosis of viral co-infections. Our device integrates nucleic acid amplification and CRISPR-based detection within a single, passively operated system. By utilizing a rapid, 10-minute sample preparation protocol and a 35-minute on-

SUBMITTER: Li X 

PROVIDER: S-EPMC12808672 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature