Exploring the mechanism of Kemofang in treating idiopathic membranous nephropathy based on LC-MS/MS combined with network pharmacology, molecular docking, and molecular dynamics simulation.

ABSTRACT: Idiopathic membranous nephropathy (IMN), an autoimmune glomerular disease, arises from in situ immune complex deposition in the glomerular subepithelial spaces, triggering complement activation and podocyte injury. Although the Kemo Formula shows therapeutic potential for IMN, its mechanisms remain unclear. This study employed LC-MS/MS, network pharmacology, molecular docking, and dynamic simulations to elucidate the mechanism of action. LC-MS/MS and the TCMSP database identified 83 bioactive components from 267 chemicals detected in the Kemo Formula. Using PubChem, Swiss Target Prediction, and GeneCards, 827 drug targets and 2581 IMN-related targets were screened, yielding 336 overlapping targets linked to 81 components. Network analysis prioritized 15 key components ( baicalein and querc

SUBMITTER: Yu H 

PROVIDER: S-EPMC12808706 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature