Peripheral Immune Cell Infiltration in the Hippocampus of Sepsis Mice.

ABSTRACT: Peripheral immune cell infiltration plays a key role in the excessive intracranial inflammatory response during sepsis, contributing to cognitive dysfunction and increased mortality. This study aims to elucidate the immune inflammatory status of hippocampal tissue in septic mice, providing potential targets for treatment and prevention. Male C57BL/6J mice underwent cecal ligation and puncture (CLP) or sham surgery. Twenty-four hours post-surgery, hippocampal tissues were harvested for transcriptome sequencing and immunofluorescence analysis. Immune cell infiltration was assessed using the xCell package, and structural changes in brain microvessels were evaluated by transmission electron microscopy. Flow cytometry and immunofluorescence confirmed immune cell presence in the hippocampus. Hub

SUBMITTER: Zhang H 

PROVIDER: S-EPMC12808816 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature