Integrated proteomic and metabolomic analyses reveal testicular metabolic mechanisms underlying sperm quality in drakes.

ABSTRACT: This study aimed to analyze the testicular metabolic mechanisms associated with the sperm quality of drakes from proteomic and metabolomic perspectives. Testicular tissues were collected from 12 drakes (six with significantly high and six with significantly low sperm quality) to evaluate sperm quality parameters. Ultra-deep quantitative proteomics and ultra-performance liquid chromatography-tandem mass spectrometry were employed to analyze differences in testicular proteomics and metabolites between groups. Enrichment analysis of differentially expressed proteins (DEPs) and metabolites (DEMPs) in testicular tissues was performed to identify key pathways associated with sperm quality. In total, 153 DEPs (65 upregulated, 88 downregulated) and 98 DEMPs (15 upregulated, 83 downregulated) were

SUBMITTER: Zhu C 

PROVIDER: S-EPMC12808949 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature