Synergistic aligned neuronal and vascular growth inside 3D-PEG-Anisogels utilizing a triple-co-culture.

ABSTRACT: Over the past few decades, researchers in tissue engineering and regenerative medicine have developed numerous strategies for cell growth and regeneration, both in vitro and in vivo. However, these strategies often focus on only one specific biomaterial, cellular composition, or molecular guidance cue, while their combination could lead to undesired cross-interactions. This study aims to understand the combined effects of several design parameters on joint neuro-vascular regeneration, including hydrogel stiffness, cell adhesive molecules, media conditions, and anisotropic guiding elements. We used a fully synthetic PEG-hydrogel system to establish a tri-culture of dorsal root ganglia from chicken embryos, human umbilical vein endothelial cells, and human mesenchymal stem cells. All cell ty

SUBMITTER: Bastard C 

PROVIDER: S-EPMC12809139 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature