A streamlined method for systematic, high resolution in situ analysis of mRNA distribution in plants.

ABSTRACT:

Background

In situ hybridisation can provide cellular, and in some cases sub-cellular, resolution of mRNA levels within multicellular organisms and is widely used to provide spatial and temporal information on gene expression. However, standard protocols are complex and laborious to implement, restricting analysis to one or a few genes at any one time. Whole-mount and reverse transcriptase-PCR (RT-PCR) based protocols increase throughput, but can compromise both specificity and resolution. With the advent of genome-wide analysis of gene expression, there is an urgent need to develop high-throughput in situ methods that also provide high resolution.

Results

Here we describe the development of a method for performing high-throughput in situ hybridisations that retains both the

SUBMITTER: Drea S 

PROVIDER: S-EPMC1280931 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature