Venous Endothelial Cells Promote Osteoblast Differentiation More Effectively Than Arterial Cells via TGF-β/BMP9 and Notch Pathway-Related Gene Expression.

ABSTRACT: The coupling between angiogenesis and osteogenesis is a key determinant of skeletal homeostasis, yet the influence of endothelial cell origin on osteoblast differentiation remains underexplored. Here, we investigated how venous (HUVECs) and arterial (HCAECs) endothelial cells differentially modulate the osteogenic phenotype of human osteoblasts via paracrine signaling. To better address this issue, the conditioned media (CM) from HUVECs significantly enhanced osteoblast differentiation, as evidenced by increased alkaline phosphatase activity, upregulation of canonical markers such as Runx2, Osterix, and Osteocalcin, and activation of matrix mineralization genes (Tnap, Bsp, Col1a1). In contrast, CM from HCAECs induced a markedly weaker response. qPCR analysis revealed that HUVEC-CM robustly

SUBMITTER: Fernandes CJC 

PROVIDER: S-EPMC12809378 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature