Extracellular vesicles from ovarian cancer tumor spheroids harbor disease-related and survival-associated proteins.

ABSTRACT: Aim: Extracellular vesicles (EVs) play a pivotal role in tumor progression, influencing the tumor microenvironment. Despite significant research, the targeted analysis of EVs directly derived from primary tumors remains limited, particularly in ovarian cancer. The majority of existing studies have focused on EVs derived from peritoneal fluid (ascites), which encompasses contributions from different cell types. This study aims to isolate and characterize EVs secreted specifically by ovarian cancer spheroids derived from primary patient ascites. Methods: A three-dimensional cell culture model was employed to cultivate tumor spheroids in a defined medium, with EVs purified via differential ultracentrifugation and size-exclusion chromatography. Purified EVs were characterized by

SUBMITTER: Preußer C 

PROVIDER: S-EPMC12809391 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature