Preanalytical framework for routine clinical use of liquid biopsies: combining EVs and cfDNA.

ABSTRACT: Aim: Liquid biopsies hold significant potential for the minimally invasive diagnosis of tumors and other diseases. While the clinical application of cell-free DNA (cfDNA) methodologies is emerging, the implementation of tumor-derived extracellular vesicles (EVs) as validated biomarkers is hindered by substantial preanalytical variations. In this work, we standardized the preanalytical procedures of blood collection for subsequent serial isolation of plasma cfDNA and EVs from a single blood collection tube. Methods: We compared the impact of blood preservation tubes and storage to enable proteomic profiling of resulting EVs in addition to cfDNA extraction and sequencing. Following a stringent method of large EV (lEV) and small EV (sEV) isolation, consisting of differential ult

SUBMITTER: Simon NK 

PROVIDER: S-EPMC12809394 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature