Limiting the effects of radiation damage in MicroED through dose selection during data processing.

ABSTRACT: Microcrystal electron diffraction (MicroED), also known as three-dimensional electron diffraction (3D ED), allows the collection of diffraction data from submicrometre-sized crystals under low electron-dose conditions. Despite having several advantages over conventional X-ray crystallographic techniques, susceptibility to radiation damage is a great challenge that remains to be solved in MicroED. Similar to X-ray crystallography, radiation damage to the macromolecular crystal structures in MicroED manifests in two forms: global damage that affects the overall order of the crystal lattice and site-specific damage that affects highly sensitive residues and moieties in macromolecules. Traditionally, the unit e^- Å^-2 has been used for electron-dose estimations, which does

SUBMITTER: Bwanika HC 

PROVIDER: S-EPMC12809500 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature