RNA G-quadruplexes promote codon repeat-associated ribosomal frameshifting in human genes.

ABSTRACT: Programmed ribosomal frameshifting (PRF), a translational recoding process previously considered as a rare event in eukaryotes, has recently been demonstrated to occur extensively in humans. We have shown that codon repeats function as the signals for the production of trans-frame proteins in various human tissues. However, the molecular mechanism underlying this recoding process, termed codon repeat-associated ribosomal frameshifting (CRFS), remains largely unknown. In this study, we developed a reporter system by incorporating the CRFS sequence from histone deacetylase 1 (HDAC1), which directs efficient +1 ribosomal frameshifting at (UAC)3 repeats. Through a whole-genome CRISPR screening, we identified RBM4, an RNA-binding protein that interacts with RNA G-quadruplexes (rG4s), as an enha

SUBMITTER: Li X 

PROVIDER: S-EPMC12809536 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature