ModuloStat: An Internet of Things' Path to Continuous Cultures in Mini-Bioreactors.

ABSTRACT: In continuous culture, a population of microorganisms is propagated in a stable environment over many generations. This is particularly relevant for experimental evolution and metabolic studies. However, continuous culture protocols are difficult to implement, so they are not commonly used in microbiology laboratories. Here, we present the ModuloStat, a modular, open-source framework that facilitates continuous culture in mini-bioreactors. The ModuloStat system is grounded on digital fabrication tools easily accessible in FabLabs and programmable electronics. Maintaining a culture is divided into tasks assigned to dedicated printed circuit boards with a microcontroller connected to a Wi-Fi network. According to Internet of Things principles, each board operates a set of sensors and actuato

SUBMITTER: Guerin C 

PROVIDER: S-EPMC12809586 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature