Light-Mediated Insulin and Amylin Secretion: Influence of Irradiation Parameters in Optogenetically Modified Mouse-Pancreatic β Cells.

ABSTRACT: Optogenetics offers a promising avenue for controlled insulin secretion in diabetes management using genetically modified cells. However, the influence of irradiation parameters on the co-secreted hormone amylin remains largely unexplored. This study examined how blue light power densities (50, 100, and 200 mW/cm²) under continuous and intermittent exposure affect hormonal secretion in channelrhodopsin-2 expressing mouse β cells. Results showed that 10 s of continuous exposure at 50 mW/cm² increased insulin and amylin secretion by ~25% and 40%, respectively. Introducing intermittent dark intervals further enhanced secretion by ~35% for insulin and 70% for amylin at 50 and 100 mW/cm² respectively, correlating with intracellular calcium changes. Notably, ligh

SUBMITTER: Maharwal N 

PROVIDER: S-EPMC12809619 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature