Engineered Reactive Interfaces Enable Mass Spectrometry Imaging of Multiple Thiols for Decoding PFOS-Induced Redox Dysregulation.

ABSTRACT: Spatial profiling of multiple thiols shows great significance in elucidating the redox status across tissue microregions and understanding the molecular mechanisms of oxidative stress injury. Traditional matrix-assisted laser desorption/ ionization mass spectrometry imaging (MALDI MSI) relies on chemical derivatization for thiol visualization, but multistep derivatization protocols and nonspecific matrix-adduct formation compromise both detection sensitivity and spatial mapping fidelity. Herein, we engineer a reactive interface-assisted chemical derivatization platform for sensitive assessment of multiple thiols in various tissues via forming the "matrix-tissue-interface" sandwich structure. Reactive interface that predeposited with N-(9-Acridinyl) maleimide (NAM) probes enables pro

SUBMITTER: Xu H 

PROVIDER: S-EPMC12809638 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature