A single-sEV analysis identifies plasma EPCAM⁺ sEVs as a biomarker for early diagnosis and monitoring postoperative remission of thyroid cancer.

ABSTRACT: Aim: Small extracellular vesicles (sEVs) are promising noninvasive biomarkers for several malignancies, including thyroid carcinoma (TC). However, their heterogeneity is frequently overlooked in bulk-level analyses. Methods: Plasma samples from TC and healthy controls (HC) were collected for a proximity-dependent barcoding assay (PBA) to identify plasma sEV biomarkers at the single-sEV level. We screened the potential biomarkers using the Panel260 (the panel that detects 260 proteins) of PBA in Cohort 1, and validated them using Panel550 (the panel that detects 550 proteins) in Cohort 2. Results: Plasma exosome counts were significantly elevated in TC compared with those in HC in both Cohort 1 and Cohort 2. Receiver operating characteristic curve analysis showed that s

SUBMITTER: Yu S 

PROVIDER: S-EPMC12809679 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature