5-hydroxymethylcytosine signature in plasma extracellular vesicle DNA as a diagnostic molecular biomarker for precancerous lesions of gastric cancer.

ABSTRACT: Aim: Precancerous lesions of gastric cancer (PLGC) represent a critical window for prevention. Developing non-invasive tools that can reliably detect these lesions is therefore a prerequisite for lowering gastric-cancer incidence. Recent work has highlighted the diagnostic promise of plasma extracellular vesicle DNAs (evDNAs) and the 5-hydroxymethylcytosine (5hmC)-Seal epigenomic platform. Here we profiled genome-wide 5hmC patterns in circulating evDNA to discover biomarkers and build a classification model. Methods: We performed whole-genome 5hmC-Seal on plasma evDNAs from 67 PLGC patients and 67 healthy individuals. By identifying trend-expressed differentially hydroxymethylated regions (DhMRs), we used machine learning algorithms to screen for diagnostic biomarkers of PLGC

SUBMITTER: Chen H 

PROVIDER: S-EPMC12809687 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature