Patient Plasma-Based Immunoproteomics Reveals Novel Echinococcus granulosus Antigens for Diagnosis of Cystic Echinococcosis.

ABSTRACT: Cystic echinococcosis (CE), a parasitic disease caused by Echinococcus granulosus (Eg), remains prevalent in underdeveloped pastoral regions. Current diagnostic methods for CE primarily rely on imaging techniques, whereas serological tests still require significant improvement. To address this challenge, we have developed an immunoproteomics workflow to identify novel diagnostic Eg antigens. Our approach involved extracting proteins from CE surgical tissues, which were then recognized by patient plasma through immunoblotting and subsequently identified using mass spectrometry. Applying stringent criteria to evaluate Eg protein antigenicity, we selected 25 candidates for expression, and 18 recombinant proteins demonstrated enhanced immunoreactivity with CE patient plasma. Further validation

SUBMITTER: Zhang C 

PROVIDER: S-EPMC12809694 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature