Engineered atopic dermatitis models for recreating hypoxic conditions in atopic dermatitis microenvironments.

ABSTRACT: Atopic dermatitis (AD) is a chronic inflammatory condition with severe itching. The complex physiology and diverse pathogenesis of AD complicate the prediction of clinical outcomes. Therefore, it is essential to develop preclinical models closely mimicking features of AD. Herein, gelatin-based in situ crosslinkable hydrogel AD models are engineered replicating the characteristics of AD tissue. First, public data of patients with AD is used to confirm the following, using single-cell RNA sequencing analysis: (1) collagen type VI alpha 5 chain (COL6A5+) fibroblast expression in patient tissues, (2) cell interaction with dorsal root ganglions that induce itching, and (3) overexpression of hypoxia-related factors in AD tissues. Based on these characteristics, an artificial AD model is d

SUBMITTER: Na KS 

PROVIDER: S-EPMC12809753 | biostudies-literature | 2026 May

REPOSITORIES: biostudies-literature