Haemanthidine-Containing Alkaloid Fraction from <i>Crinum scabrum</i> as a Natural Therapeutics for Chagas Disease.

ABSTRACT: Crinum scabrum, an underexplored species and potential source of the bioactive alkaloid haemanthidine, was investigated. A crude methanolic extract was prepared and subjected to acid-base extraction to obtain an alkaloid-enriched fraction. Four alkaloids were isolated and purified; structural characterization via ¹H and ¹³C NMR spectroscopy, coupled with literature comparison, identified them as haemanthidine/6-epi-haemanthidine (C1/2), crinamine (C3), and hamayne (C4). The extracts and purified compounds were screened against Trypanosoma cruzi (amastigote and trypomastigote forms) and Vero host cells. Fraction III exhibited potent activity against intracellular amastigotes (IC₅₀ = 0.88 ± 0.08 μg mL^-1

SUBMITTER: Pardo JB 

PROVIDER: S-EPMC12809786 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature