ATR-FTIR Spectroscopy for Detection of Anti-interferon-Gamma Autoantibodies.

ABSTRACT: Introduction: Adult-onset immunodeficiency (AOID) unrelated to HIV is commonly linked to anti-interferon-gamma autoantibodies (AIGAs), predisposing patients to life-threatening opportunistic infections. Current AIGA detection methods are time-consuming and resource intensive. This study aimed to develop and validate an attenuated total reflectance-Fourier transform infrared (ATR-FTIR) technique for detecting and differentiating AIGA-positive patients. Methods: We recruited individuals into three groups: AIGA-positive patients (n = 45), AIGA-negative patients (n = 15), and healthy controls (n = 15). Heparinized plasma or serum samples underwent ATR-FTIR spectroscopy. Spectral profiles were examined using principal component analysis (PCA) and partial least

SUBMITTER: Hongsa C 

PROVIDER: S-EPMC12809796 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature