Establishment of a novel brainstem ischemic dysphagia model: single-cell sequencing reveals the molecular mechanisms underlying mPES intervention.

ABSTRACT:

Objective

Previous animal models of post-stroke dysphagia (PSD) have limitations-these models are primarily induced by cortical strokes. Compared to cortical strokes, brainstem strokes are more likely to cause dysphagia, and the pathological mechanisms underlying dysphagia differ by focal infarction location. This study aimed to create a novel rat dysphagia model via brainstem ischemia (BSI) and explore modified pharyngeal electrical stimulation (mPES) therapy.

Methods

Rat brainstem ischemia was induced by photochemical embolization, confirmed by MRI and pathology. Swallowing function was assessed using Videofluoroscopic Swallowing Study (VFSS), while motor and neurobehavioral changes were evaluated through behavioral tests. Post-mPES treatment, VFSS was conducted to evaluat

SUBMITTER: Tian Y 

PROVIDER: S-EPMC12809966 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature