Virus Capsid Modifications Accompanying Inactivation during Iron Electrocoagulation Revealed by Proteomics, Infrared Spectroscopy, and Molecular Modeling.

ABSTRACT: Society's drinking water needs are increasingly met by reusing municipal wastewater, necessitating high virus Log₁₀ Reduction Values (LRVs). Concurrently, electrified processes are gaining prominence for water/wastewater treatment. Herein, we report that electrocoagulation with a low-carbon steel anode and graphite cathode at pH 6.5 and 5.5 and iron dose of 20 mg/L reduced the MS2 coliphage below detection limits (LRVs ≳6.7) in just 11.5 min. Matrix Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) of electrocoagulated viruses revealed single, double, and triple oxygen adducts and a negative mass shift peak in its coat protein without cleavage/scission. Density functional theory calculations coupled with computational spatial interaction

SUBMITTER: Verma A 

PROVIDER: S-EPMC12810256 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature