Versatile Microfluidics Platform for Enhanced Multitarget Super-Resolution Microscopy.

ABSTRACT: DNA-based Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) is a powerful variant of single-molecule localization microscopy (SMLM) that overcomes the limitations of photobleaching, offers flexible fluorophore selection, and enables fine control of imaging parameters through tunable on- and off-binding kinetics. Its most distinctive feature is its capacity for multiplexing, typically implemented through sequential imaging of targets using an Exchange-PAINT. This technique involves assigning orthogonal DNA strands to different targets within a sample and then sequentially adding and removing complementary imager strands that are specific to only one target at a time. However, manual Exchange-PAINT workflows are often inefficient, prone to drift and variability, and lack rep

SUBMITTER: Basak S 

PROVIDER: S-EPMC12810472 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature