Assessing the problem of excitation light scattering in high-viscosity jets used in serial crystallography sample delivery.

ABSTRACT: Serial crystallography for time-resolved structural studies of light-triggered reactions often employs high-viscosity jets to deliver crystals into an X-ray beam. A potential complication is that pump light can scatter within the jet to unintentionally irradiate yet-to-be-probed portions of the jet - a problem known as light contamination. Importantly, by transporting light out of the nominal interaction region, light scattering can reduce the effective irradiation energy density experienced by the diffracting crystal. This issue, which can even jeopardize an experiment, has proven rather controversial. To provide direct insight, we performed custom femtosecond transient absorption experiments with spatially displaced pump and probe beams directed onto actual jets under realistic experimen

SUBMITTER: Nizinski S 

PROVIDER: S-EPMC12810516 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature