Dual-mode microfluidic immunostaining device for diagnostic biomarkers detection and tumor microenvironment evaluation.

ABSTRACT: The limited availability of tissue samples from rare tumors poses a major barrier to advances in precise diagnosis, prognostic evaluation, and therapeutic research-challenges exemplified by primary central nervous system diffuse large B cell lymphoma (PCNS-DLBCL). Here, we developed a dual-mode microfluidic immunostaining (Dumi) device, which integrates diagnostic and research workflows into a single, automated platform, reducing tissue section consumption by more than 90%. Using just one to two slides, it enables both regional detection of biomarkers for diagnostic subtyping (≤16) and construction of multiplex tumor microenvironment (TME) maps. Joint analysis of multiregion diagnostic biomarkers in the TME map indicates that tumor cell subpopulations defined by specific diagnostic biomark

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12810582 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature