Shedding Light on PFAS Dark Matter Using a Novel GC-HRMS Approach.

ABSTRACT: With thousands of documented perfluoroalkyl and polyfluoroalkyl substances (PFAS), novel methods are needed to comprehensively characterize these fluorinated compounds in the environment, biota, and humans. Despite being the most common approach, liquid chromatography high-resolution mass spectrometry (LC-HRMS) only covers a fraction of PFAS. To address this gap, we developed a nontargeted gas chromatography high-resolution mass spectrometry (GC-HRMS) workflow to extend coverage of the PFAS chemical space, developing and incorporating the most extensive GC HRMS PFAS libraries to date covering over 1,900 electron ionization (EI) and positive chemical ionization (PCI) predicted and experimental spectra. Five environmental and biological matrices were assessed: aqueous film forming foam (AFFF

SUBMITTER: Koelmel JP 

PROVIDER: S-EPMC12810681 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature