Impact of Copper on the Physiology and Transcriptome of Methylosinus Trichosporium OB3b Grown on Either Methane or Methanol.

ABSTRACT: Methylosinus trichosporium OB3b is a model methanotroph that converts methane to CO₂ with methanol, formaldehyde, and formate as intermediates. Here we show that copper has a significant impact on the ability of Msn. trichosporium OB3b to grow on methanol, that is, growth consistently occurred on methanol in the absence of copper but not in its presence. Growth on methanol in the presence of copper, however, occurred in the presence of MOPS buffer that stabilized pH. Examination of transcriptome profiles, NADH/NAD ratios, and formate production indicates that growth disruption in the presence of copper was likely caused by a confluence of (1) overgeneration of reducing power from methanol oxidation; (2) formate accumulation leading to acidification of the growth medium and inact

SUBMITTER: Peng P 

PROVIDER: S-EPMC12811070 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature