Anti-cryptosporidial efficacy of tricyclic carbazole aminoalcohols in vitro and in vivo.

ABSTRACT: Cryptosporidiosis is a major cause of diarrheal disease in humans and animals, yet there are no fully effective drugs, particularly for immunocompromised patients. Carbazole aminoalcohols (CAAs) are a relatively unexplored chemical class with reported broad-spectrum antiparasitic activities. Here, we systematically evaluated 36 CAA derivatives for anti-cryptosporidial efficacy. In vitro screening using a 44-h Cryptosporidium parvum infection assay identified eight compounds with low-micromolar EC₅₀ values (1.53-6.86 μM). The three most potent hits (H1402 at 25 mg/kg/d, and YFM1 and YFM3 at 15 mg/kg/d) were selected for in vivo evaluation in a chronic C. tyzzeri mouse model. Daily oral treatment with these compounds for 7 days reduced fecal oocyst shedding by 55.9-59.1 % compared

SUBMITTER: Liu M 

PROVIDER: S-EPMC12811158 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature