Molecular identification of potentially pathogenic free-living amoebae in environmental samples from urban areas of Kerman, Southeastern Iran.

ABSTRACT: Free-living amoebae (FLA) are widely distributed protozoans in various habitats. Some genera of these amoebae have the potential to be pathogenic to humans. The aim of this study was to identify the distribution of different FLA species in various environmental sources in Kerman, southeastern Iran. A total of 141 samples, including city fountains, tap water, and soil samples, were collected from various regions of the city. After filtration and cultivation on non-nutrient agar, molecular analysis was performed using PCR-sequencing with genus specific primers for Acanthamoeba, Balamuthia, Vahlkampfidae, and Hartmannella. PCR- sequencing fountain water samples revealed the presence of Acanthamoeba, Hartmannella and Naegleria species. Among the sequen

SUBMITTER: Ghasemi AA 

PROVIDER: S-EPMC12811234 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature