Metabolomic and functional profiling of Lactiplantibacillus plantarum strains reveals distinct probiotic, immunomodulatory, and antimicrobial signatures.

ABSTRACT: Lactiplantibacillus plantarum strains are increasingly recognized for their combined probiotic and antimicrobial activities, offering potential applications in gut health management and pathogen control. This study characterized the intracellular (Met-Int) and extracellular (Met-Ext) metabolomic profiles of L. plantarum UTNGt2 (Gt2), UTNGt3 (Gt3), and UTNGt28L (Gt28L) isolated from tropical fruits and evaluated their probiotic, antimicrobial, cytotoxic, and immunomodulatory properties in vitro. Metabolomic profiling was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with a SWATH (Sequential Windowed Acquisition of All Theoretical Fragment Ion Mass Spectra) acquisition method. Cytotoxicity and cell viability were assessed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-d

SUBMITTER: Tenea GN 

PROVIDER: S-EPMC12811343 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature