Renal podocyte senescence in human obesity detected by urinary extracellular vesicles.

ABSTRACT:

Background

Obesity is a major risk factor for kidney dysfunction, with cellular senescence and senescence-associated secretory phenotype (SASP) activation contributing to early kidney injury. Urinary extracellular vesicles (uEVs) provide a non-invasive approach to assess cellular stress. We hypothesised that obesity induces podocyte senescence detectable by podocyte-derived uEVs.

Methods

We recruited 28 obese (OB) individuals and 16 healthy volunteers (HV). Of these, uEVs from 21 OB (OB-1) and 10 HV (HV-1) were analysed for tubular-derived (urat1⁺, uromodulin⁺, or prominin⁺) or podocyte-specific (PODXL⁺) uEVs bearing markers of senescence (p16) and SASP (MCP-1) using flow cytometry. Their correlations with metabolic and urinary ren

SUBMITTER: Zhang L 

PROVIDER: S-EPMC12811474 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature