Identification of biofilm-associated genes in <i>Arcobacter butzleri</i>.

ABSTRACT: Arcobacter butzleri is an emerging zoonotic and foodborne pathogen isolated from human diarrhoeal samples and environmental sources. Despite its increasing clinical relevance, functional studies in this species have been limited by the lack of effective genetic tools. In this study, we report the first successful application of random transposon mutagenesis using the EZ-Tn5 transposome system in three A. butzleri strains. Transformation efficiency was found to be strain-dependent, with only one strain (P8) yielding enough mutants for phenotypic screening. A total of 56 mutants were characterised with 29 different disrupted genes, all exhibiting significantly reduced biofilm formation, and ten strains showing reduced or abolished motility. Furthermore, the phenotype of some mu

SUBMITTER: Salazar-Sanchez A 

PROVIDER: S-EPMC12811638 | biostudies-literature | 2026 Jun

REPOSITORIES: biostudies-literature