Identification of Escherichia coli genes that are specifically expressed in a murine model of septicemic infection.

ABSTRACT: Identification and characterization of bacterial genes that are induced during the disease process are important in understanding the molecular mechanism of disease and can be useful in designing antimicrobial drugs to control the disease. The identification of in vivo induced (ivi) genes of an Escherichia coli septicemia strain by using antibiotic-based in vivo expression technology is described. Bacterial clones resistant to chloramphenicol in vivo were recovered from the livers of infected mice. Most of the ivi clones were sensitive to chloramphenicol when grown in vitro. Using reverse transcription-PCR, it was demonstrated that selected ivi clones expressed cat in the livers of infected mice but not during in vitro growth. A total of 750 colonies were recovered after three successive r

SUBMITTER: Khan MA 

PROVIDER: S-EPMC128117 | biostudies-literature | 2002 Jul

REPOSITORIES: biostudies-literature