Single-Cell Transcriptome Profiling Reveals the Immune Dysregulation Characteristics of Mice Infected With Brucella abortus.

ABSTRACT:

Background

Brucellosis poses a significant threat to animal and human health globally. However, how Brucella subverts the immune response to establish persistent infections remains unclear.

Methods

We utilized single-cell RNA sequencing (scRNA-seq) to decipher the immune landscape of mice infected with Brucella abortus. Flow cytometry, a transgenic cell line and mouse, and antibody blockage were utilized to explore the relevant mechanisms.

Results

Brucella infection induced significant changes in the composition and signaling pathways of immune cells, and flow cytometry analysis further confirmed the scRNA-seq data. An in-depth analysis of macrophages, the main target cell for Brucella, demonstrated activation of type I interferon (IFN) and type II IFN signaling, tumo

SUBMITTER: Zhang G 

PROVIDER: S-EPMC12811882 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature